Component of the endosomal sorting complex required for transport II (ESCRTII), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs.
The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation.
The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex.
The ESCRT-II complex may also play a role in transcription regulation by participating in derepression of transcription by RNA polymerase II, possibly via its interaction with ELL.
Required for degradation of both endocytosed EGF and EGFR, but not for the EGFR ligand-mediated internalization.
It is also required for the degradation of CXCR4.
Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).
Applications:Suitable for use in ELISA and Immunofluorescence.
Other applications not tested.
Recommended Dilutions:Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a liquid in PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol
Specificity：Recognizes human SNF8.
Isotype：IgG
Calc Applications Abbrev：E IF
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa1-258 of human SNF8. Subcellular Location: Cytoplasm, Endosome membrane, Nucleus, Late endosome membrane
