Functions as an intracellular leucine sensor that negatively regulates theTORC1 signaling pathway through the GATOR complex.
In absence of leucine, binds the GATOR subcomplex GATOR2 and prevents TORC1 signaling (PubMed:18692468, PubMed:25263562, PubMed:25457612,PubMed:26449471, PubMed:26612684, PubMed:26586190).
Binding of leucine to SESN2 disrupts its interaction with GATOR2 thereby activating the TORC1 signaling pathway (PubMed:26449471, PubMed:26586190).
This stressinducible metabolic regulator also plays a role in protection against oxidative and genotoxic stresses.
May negatively regulate protein translation in response to endoplasmic reticulum stress, via TORC1 (PubMed:24947615).
May positively regulate the transcription by NFE2L2 of genes involved in the response to oxidative stress by facilitating the SQSTM1-mediated autophagic degradation of KEAP1 (PubMed:23274085).
May also mediate TP53 inhibition of TORC1 signaling upon genotoxic stress (PubMed:18692468).
Has analkylhydroperoxide reductase activity born by the N-terminal domain of theprotein (PubMed:26612684).
Was originally reported to contribute to oxidative stress resistance by reducing PRDX1 (PubMed:15105503).
However, this could not be confirmed (PubMed:19113821).
Applications:Suitable for use in ELISA and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Specificity：Recognizes human SESN2.
Isotype：IgG
Calc Applications Abbrev：E IF
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa42-61 from human Sestrin-2 protein.
