Protease that catalyzes two essential functions in the SUMO pathway:processing of full-length SUMO1, SUMO2 and SUMO3 to their mature forms and deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins.
Deconjugates SUMO1 from HIPK2.
Deconjugates SUMO1 from HDAC1 and BHLHE40/DEC1, which decreases its transcriptional repression activity.
Deconjugates SUMO1 from CLOCK, which decreases its transcriptional activation activity.
Deconjugates SUMO2 from MTA1.
Desumoylates CCAR2 which decreases its interaction with SIRT1 (PubMed:25406032).
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:5000Immunohistochemistry: 1:20-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Specificity：Recognizes human SENP1. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant protein corresponding to aa63-214 from human Sentrin-specific protease 1 protein.
