The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination.
RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing.
RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB.
Binds both single- and double-stranded DNA (dsDNA).
Binds preferentially to supercoiled rather than to relaxed dsDNA.
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：E. coli
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Specificity：Recognizes E. coli ruvA.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Ec
Immunogen：Recombinant protein corresponding to aa1-203 from E. coli Holliday junction ATP-dependent DNA helicase RuvA protein.
