Catalyzes uridine to pseudouridine isomerization (pseudouridylation) of different mitochondrial RNA substrates.
Acts on position 1397 in 16S mitochondrial ribosomal RNA (16S mt-rRNA).
This modification is required for the assembly of 16S mt-rRNA into a functional mitochondrial ribosome (PubMed:27974379).
Acts on position 39 in mitochondrial tRNA(Phe) (PubMed:28082677).
As a component of a functional protein-RNA module, consisting of RCC1L, NGRN, RPUSD3, RPUSD4, TRUB2, FASTKD2 and 16S mt-rRNA, controls 16S mtrRNA abundance and is required for intra-mitochondrial translation (PubMed:27667664).
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:20-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Specificity：Recognizes human RPUSD4.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa41-240 from human mitochondrial RNA pseudouridine synthase RPUSD4 protein.
