Protein kinase which is a key regulator of actin cytoskeleton and cell polarity.
Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A.
Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing.
Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress.
Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability.
Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation.
Required for centrosome positioning and centrosome-dependent exit from mitosis.
Plays a role in terminal erythroid differentiation.
May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles.
Promotes keratinocyte terminal differentiation.
Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000Immunohistochemistry: 1:200-1:500Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Specificity：Recognizes human ROCK1.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa405-668 from human Rho-associated protein kinase 1 protein.
