84-6085-91　RBM4 100ug　574412

特徴

• RNA-binding factor involved in multiple aspects of cellular processes like alternative splicing of pre-mRNA and translation regulation.
• Modulates alternative 5'-splice site and exon selection.
• Acts as a muscle cell differentiation-promoting factor.
• Activates exon skipping of the PTB pre-mRNA during muscle cell differentiation.
• Antagonizes the activity of the splicing factor PTBP1 to modulate muscle cell-specific exon selection of alpha tropomyosin.
• Binds to intronic pyrimidine-rich sequence of the TPM1 and MAPT pre-mRNAs.
• Required for the translational activation of PER1 mRNA in response to circadian clock.
• Binds directly to the 3'-UTR of the PER1 mRNA.
• Exerts a suppressive activity on Cap-dependent translation via binding to CU-rich responsive elements within the 3'UTR of mRNAs, a process increased under stress conditions or during myocytes differentiation.
• Recruits EIF4A1 to stimulate IRES-dependent translation initiation in respons to cellular stress.
• Associates to internal ribosome entry segment (IRES) in target mRNA species under stress conditions.
• Plays a role for miRNA-guided RNA cleavage and translation suppression by promoting association of AGO2-containing miRNPs with their cognate target mRNAs.
• Associates with miRNAs during muscle cell differentiation.
• Binds preferentially to 5'-CGCGCG[GCA]-3' motif in vitro.
• Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:5000Immunohistochemistry: 1:20-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.