84-6073-39　PTK2B 100ug　573792

特徴

• Non-receptor protein-tyrosine kinase that regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading and bone remodeling.
• Plays a role in the regulation of the humoral immune response, and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells.
• Required for normal macrophage polarization and migration towards sites of inflammation.
• Regulates cytoskeleton rearrangement and cell spreading in T-cells, and contributes to the regulation of T-cell responses.
• Promotes osteoclastic bone resorption; this requires both PTK2B/PYK2 and SRC.
• May inhibit differentiation and activity of osteoprogenitor cells.
• Functions in signaling downstream of integrin and collagen receptors, immune receptors, G-protein coupled receptors (GPCR), cytokine, chemokine and growth factor receptors, and mediates responses to cellular stress.
• Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates.
• Regulates numerous signaling pathways.
• Promotes activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade.
• Promotes activation of NOS3.
• Regulates production of the cellular messenger cGMP.
• Promotes activation of the MAP kinase signaling cascade, including activation of MAPK1/ERK2, MAPK3/ERK1 and MAPK8/JNK1.
• Promotes activation of Rho family GTPases, such as RHOA and RAC1.
• Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation.
• Acts as a scaffold, binding to both PDPK1 and SRC, thereby allowing SRC to phosphorylate PDPK1 at 'Tyr-9, 'Tyr-373', and 'Tyr-376'.
• Promotes phosphorylation of NMDA receptors by SRC family members, and thereby contributes to the regulation of NMDA receptor ion channel activity and intracellular Ca(2+) levels.
• May also regulate potassium ion transport by phosphorylation of potassium channel subunits.
• Phosphorylates SRC; this increases SRC kinase activity.
• Phosphorylates ASAP1, NPHP1, KCNA2 and SHC1.
• Promotes phosphorylation of ASAP2, RHOU and PXN; this requires both SRC and PTK2/PYK2.
• Applications:Suitable for use in ELISA, Immunohistochemistry and Immunofluorescence.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry: 1:20-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.