Multifunctional transcription factor with different regions mediating its different effects.
Acts by binding (via its C-terminal domain) to sequences related to the consensus octamer motif 5'-ATGCAAAT-3' in the regulatory regions of its target genes.
Regulates the expression of specific genes involved in differentiation and survival within a subset of neuronal lineages.
It has been shown that activation of some of these genes requires its N-terminal domain, maybe through a neuronal-specific cofactor.
Ativates BCL2 expression and protects neuronal cells from apoptosis (via the N-terminal domain).
Induces neuronal process outgrowth and the coordinate expression of genes encoding synaptic proteins.
Exerts its major developmental effects in somatosensory neurons and in brainstem nuclei involved in motor control.
Stimulates the binding affinity of the nuclear estrogene receptor ESR1 to DNA estrogen response element (ERE), and hence modulates ESR1-induced transcriptional activity.
May positively regulate POU4F2 and POU4F3.
Regulates dorsal root ganglion sensory neuron specification and axonal projection into the spinal cord.
Plays a role in TNFSF11-mediated terminal osteoclast differentiation.
Negatively regulates its own expression interacting directly with a highly conserved autoregulatory domain surrounding the transcription initiation site.
Applications:Suitable for use in FLISA.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes human POU4F1.
Isotype：IgG
Calc Applications Abbrev：FLISA
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa258-419 from human POU domain, class 4, transcription factor 1 protein.
