Exhibits PLA1/2 activity, catalyzing the calcium-independent hydrolysis of acyl groups in various phosphatidylcholines (PC) and phosphatidylethanolamine (PE).
For most substrates, PLA1 activity is much higher than PLA2 activity.
Specifically catalyzes the release of fatty acids from phospholipids in adipose tissue (By similarity).
N- and O-acylation activity is hardly detectable.
Might decrease protein phosphatase 2A (PP2A) activity.
Applications:Suitable for use in ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:20-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:50ug
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by Protein G affinity chromatography.
Form:Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Specificity:Recognizes human PLA2G16.
Isotype:IgG
Calc Applications Abbrev:E IHC
Calc Crossreactivity:Hu
Immunogen:Recombinant protein corresponding to aa1-132 from human HRAS-like suppressor 3 protein.