Peptidyl-prolyl cis/trans isomerase (PPIase) that binds to and isomerizesspecific phosphorylated Ser/Thr-Pro (pSer/Thr-Pro) motifs.
By inducingconformational changes in a subset of phosphorylated proteins, acts as amolecular switch in multiple cellular processes (PubMed:21497122,PubMed:22033920, PubMed:23623683).
Displays a preference for acidicresidues located N-terminal to the proline bond to be isomerized.
Regulates mitosis presumably by interacting with NIMA and attenuating its mitosispromoting activity.
Down-regulates kinase activity of BTK (PubMed:16644721).
Can transactivate multiple oncogenes and induce centrosome amplification, chromosome instability and cell transformation.
Required for the efficient dephosphorylation and recycling of RAF1 after mitogen activation (PubMed:15664191).
Binds and targets PML and BCL6 for degradation in a phosphorylation-dependent manner (PubMed:17828269).
Acts as a regulator of JNK cascade by binding to phosphorylated FBXW7, disrupting FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation: degradation of FBXW7 leads to subsequent stabilization of JUN (PubMed:22608923).
May facilitate the ubiquitination and proteasomal degradation of RBBP8/CtIP through CUL3/KLHL15 E3 ubiquitin-protein ligase complex, hence favors DNA double-strand repair through error-prone nonhomologous end joining (NHEJ) over error-free, RBBP8-mediated homologousrecombination (HR) (PubMed:23623683, PubMed:27561354).
Applications:Suitable for use in ELISA, Immunohistochemistry, and Immunoprecipitation
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:20-1:200Immunoprecipitation: 1:200-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Specificity：Recognizes human PIN1.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa1-163 from human peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 protein.
