Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3).
PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology.
Links G-protein coupled receptor activation to PIP3 production.
Involved in immune, inflammatory and allergic responses.
Modulates leukocyte chemotaxis to inflammatory sites and in response to chemoattractant agents.
May control leukocyte polarization and migration by regulating the spatial accumulation of PIP3 and by regulating the organization of F-actin formation and integrin-based adhesion at the leading edge.
Controls motility of dendritic cells.
Together with PIK3CD is involved in natural killer (NK) cell development and migration towards the sites of inflammation.
Participates in T-lymphocyte migration.
Regulates T-lymphocyte proliferation and cytokine production.
Together with PIK3CD participates in T-lymphocyte development.
Required for B-lymphocyte development and signaling.
Together with PIK3CD participates in neutrophil respiratory burst.
Together with PIK3CD is involved in neutrophil chemotaxis and extravasation.
Together with PIK3CB promotes platelet aggregation and thrombosis.
Regulates alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) adhesive function in platelets downstream of P2Y12 through a lipid kinase activity-independent mechanism.
May have also a lipid kinase activity-dependent function in platelet aggregation.
Involved in endothelial progenitor cell migration.
Negative regulator of cardiac contractility.
Modulates cardiac contractility by anchoring protein kinase A (PKA) and PDE3B activation, reducing cAMP levels.
Regulates cardiac contractility also by promoting beta-adrenergic receptor internalization by binding to GRK2 and by non-muscle tropomyosin phosphorylation.
Also has serine/threonine protein kinase activity
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human PIK3CG.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa438-588 from human phosphatidylinositol 4, 5-bisphosphate 3-kinase catalytic subunit gamma isoform.
