Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3).
PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology.
Involved in the activation of AKT1 upon stimulation by G-protein coupled receptors (GPCRs) ligands such as CXCL12, sphingosine 1-phosphate, and lysophosphatidic acid.
May also act downstream receptor tyrosine kinases.
Required in different signaling pathways for stable platelet adhesion and aggregation.
Plays a role in platelet activation signaling triggered by GPCRs, alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) and ITAM (immunoreceptor tyrosine-based activation motif)-bearing receptors such as GP6.
Regulates the strength of adhesion of ITGA2B/ ITGB3 activated receptors necessary for the cellular transmission of contractile forces.
Required for platelet aggregation induced by F2 (thrombin) and thromboxane A2 (TXA2).
Has a role in cell survival.
May have a role in cell migration.
Involved in the early stage of autophagosome formation.
Modulates the intracellular level of PtdIns3P (Phosphatidylinositol 3-phosphate) and activates PIK3C3 kinase activity.
May act as a scaffold, independently of its lipid kinase activity to positively regulate autophagy.
May have a role in insulin signaling as scaffolding protein in which the lipid kinase activity is not required.
May have a kinase-independent function in regulating cell proliferation and in clathrin-mediated endocytosis.
Mediator of oncogenic signal in cell lines lacking PTEN.
The lipid kinase activity is necessary for its role in oncogenic transformation.
Required for the growth of ERBB2 and RAS driven tumors.
Applications:Suitable for use in ELISA and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Specificity：Recognizes human PIK3CB.
Isotype：IgG
Calc Applications Abbrev：E IF
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa90-320 from human phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform protein.
