84-6042-41　PIK3CA 50ug　572259

特徴

• Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3).
• PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology.
• Participates in cellular signaling in response to various growth factors.
• Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF.
• Involved in signaling via insulin-receptor substrate (IRS) proteins.
• Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity.
• Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF.
• Regulates invadopodia formation through the PDPK1-AKT1 pathway.
• Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway.
• Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway.
• Also has serine-protein kinase activity.
• Applications:Suitable for use in ELISA, Immunohistochemistry and Immunofluorescence.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry: 1:20-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.