84-6038-83　PHAX 50ug　572082

特徴

• A phosphoprotein adapter involved in the XPO1-mediated U snRNA export from the nucleus.
• Bridge components required for U snRNA export, the cap binding complex (CBC)-bound snRNA on the one hand and the GTPase Ran in its active GTP-bound form together with the export receptor XPO1 on the other.
• Its phosphorylation in the nucleus is required for U snRNA export complex assembly and export, while its dephosphorylation in the cytoplasm causes export complex disassembly.
• It is recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor.
• The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
• Its compartmentalized phosphorylation cycle may also contribute to the directionality of export.
• Binds strongly to m7G-capped U1 and U5 small nuclear RNAs (snRNAs) in a sequence-unspecific manner and phosphorylation-independent manner (By similarity).
• Plays also a role in the biogenesis of U3 small nucleolar RNA (snoRNA).
• Involved in the U3 snoRNA transport from nucleoplasm to Cajal bodies.
• Binds strongly to m7G-capped U3, U8 and U13 precursor snoRNAs and weakly to trimethylated (TMG)-capped U3, U8 and U13 snoRNAs.
• Binds also to telomerase RNA.
• Applications:Suitable for use in ELISA, Western Blot and Immunofluorescence.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:5000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.