84-6034-93　PECAM1 50ug　571889

特徴

• Induces susceptibility to atherosclerosis (By similarity).
• Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions.
• Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes.
• Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes functionon apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte.
• During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore.
• The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment).
• Isoform Delta15 is unableto protect against apoptosis.
• Modulates BDKRB2 activation.
• Regulatesbradykinin- and hyperosmotic shock-induced ERK1/2 activation in humanumbilical cord vein cells (HUVEC).
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:5000Immunohistochemistry: 1:100-1:500Immunofluorescence: 1:50-1:500Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.