Inhibits pyruvate dehydrogenase activity by phosphorylation of the E1 subunit PDHA1, and thereby regulates glucose metabolism and aerobic respiration.
Can also phosphorylate PDHA2.
Decreases glucose utilization and increases fat metabolism in response to prolonged fasting, and as adaptation to a high-fat diet.
Plays a role in glucose homeostasis and in maintaining normal blood glucose levels in function of nutrient levels and under starvation.
Plays a role in the generation of reactive oxygen species (By similarity).
Applications:Suitable for use in FLISA.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes mouse Pdk3.
Isotype：IgG
Calc Applications Abbrev：FLISA
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to aa131-362 from mouse [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3, mitochondrial protein.
