A bis-(3'-5') cyclic diguanylic acid (c-di-GMP) phosphodiesterase involved in regulating the levels of c-di-GMP that control cell motility (the flagella) and adhesion (adhesive curli fimbriae).
The YhjH effect on flagella is controlled via the c-di-GMP-binding flagellar brake protein YcgR, however curli expression is not regulated via YcgR.
Forms a network with different diguanylate cyclases (DosC, YeaJ, YedQ, YegE have been identified) to regulate c-di-GMP levels.
Flagellar activity is high at low c-di-GMP levels whereas curli fimbria are induced at high c-di-GMP levels.
Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria.
Applications:Suitable for use in FLISA.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：E. coli
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes E.coli (strain K12) pdeH.
Isotype：IgG
Calc Applications Abbrev：FLISA
Calc Crossreactivity：Ec
Immunogen：Recombinant protein corresponding to aa1-255 from E.coli cyclic di-GMP phosphodiesterase PdeH protein.
