Involved in DNA damage response and in transcriptional regulation throughhistone methyltransferase (HMT) complexes.
Plays a role in early development.
In DNA damage response is required for cell survival after ionizing radiation.
In vitro shown to be involved in the homologous recombination mechanism for the repair of double-strand breaks (DSBs).
Its localization to DNA damage foci requires RNF8 and UBE2N.
Recruits TP53BP1 to DNA damage foci and, at least in particular repair processes, effective DNA damage response appears to require the association with TP53BP1 phosphorylated by ATM at 'Ser-25'.
Together with TP53BP1 regulates ATM association.
Recruits PAGR1 to sites of DNA damage and the PAGR1:PAXIP1 complex is required for cell survival in response to DNA damage; the function is probbaly independent of MLLcontaining histone methyltransferase (HMT) complexes.
Promotes ubiquitinationof PCNA following UV irradiation and may regulate recruitment of polymerase eta and RAD51 to chromatin after DNA damage.
Proposed to be involved in transcriptional regulation by linking MLL-containing histone methyltransferase (HMT) complexes to gene promoters by interacting with promoter-bound transcription factors such as PAX2.
Associates with gene promoters that are known to be regulated by KMT2D/MLL2.
During immunoglobulin class switching in activated B-cells is involved in trimethylation of histone H3 at 'Lys-4' and in transcription initiation of downstream switch regions at the immunoglobulin heavy-chain (Igh) locus; this function appears to involve the recruitment of MLLcontaining HMT complexes.
Applications:Suitable for use in ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:20-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Specificity：Recognizes human PAXIP1.
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa613-812 from human PAX-interacting protein 1.
