Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP.
Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate but with a lower efficiency.
Cannot use triazofurin monophosphate (TrMP) as substrate.
Also catalyzes the reverse reaction, i.e.
the pyrophosphorolytic cleavage of NAD(+).
For the pyrophosphorolytic activity prefers NAD(+), NADH and NaAD as substrates and degrades nicotinic acid adenine dinucleotide phosphate (NHD) less effectively.
Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NaADP(+).
Applications:Suitable for use in FLISA.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes human NMNAT2.
Isotype：IgG
Calc Applications Abbrev：FLISA
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa1-153 from human nicotinamide/nicotinic acid mononucleotide adenylyltransferase 2 protein.
