As the sensor component of the NLRP1 inflammasome, plays a crucial role in innate immunity and inflammation.
In response to pathogens and other damageassociated signals, initiates the formation of the inflammasome polymeric complex, made of NLRP1, CASP1, and possibly PYCARD.
Recruitement of proCASP1 to the inflammasome promotes its activation and CASP1-catalyzed IL1B and IL18 maturation and secretion in the extracellular milieu.
Activation of NLRP1 inflammasome is also required for HMGB1 secretion.
The active cytokines and HMGB1 stimulate inflammatory responses.
Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death (PubMed:22665479, PubMed:17418785).
May be activated by muramyl dipeptide (MDP), a fragment of bacterial peptidoglycan, in a NOD2-dependentmanner (PubMed:18511561).
Contrary to its mouse ortholog, not activated by Bacillus anthracis lethal toxin (PubMed:19651869).
It is unclear whether isoform 2 is involved in inflammasome formation.
It is not cleaved within the FIIND domain, does not assemble into specks, nor promote IL1B release (PubMed:22665479).
However, in an vitro cell-free system, it has been shown to be activated by MDP (PubMed:17349957).
Binds ATP (PubMed:11113115, PubMed:15212762).
Applications:Suitable for use in ELISA and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Specificity：Recognizes human NLRP1.
Isotype：IgG
Calc Applications Abbrev：E IF
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa1-146 from human NACHT, LRR and PYD domains-containing protein 1.
