Core subunit of the mitochondrial membrane respiratory chain NADHdehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis.
Complex I functions in the transfer of electrons from NADH to the respiratory chain.
The immediate electron acceptor for the enzyme is believed to be ubiquinone.
This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme.
It may form part of the active site crevice where NADH is oxidized.
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:50ug
Host:rabbit
Source Antibody:Drosophila melanogaster
Grade:Affinity Purified
Purity:Purified by Protein G affinity chromatography.
Form:Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Immunogen:Recombinant protein corresponding to aa28-315 from Drosophila melanogaster NADH-ubiquinone oxidoreductase 75kD subunit, mitochondrial protein.