Inhibits the transcriptional activation of Zic family proteins ZIC1, ZIC2 and ZIC3.
Retains nuclear Zic proteins ZIC1, ZIC2 and ZIC3 in the cytoplasm.
Modulates the expression from both cellular and viral promoters.
Down-regulates Tat-dependent transcription of the human immunodeficiency virus type 1 (HIV-1) LTR by interacting with HIV-1 Tat and Rev and impairing their nuclear import, probably by rendering the NLS domains inaccessible to importin-beta.
Also stimulates activation of human T-cell leukemia virus type I (HTLV-I) LTR.
Binds to the axin complex, resulting in an increase in the level of free beta-catenin.
Affects axin regulation of the WNT and JNK signaling pathways.
Applications:Suitable for use in ELISA, Immunohistochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:200-1:500Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Specificity：Recognizes human MDFIC.
Isotype：IgG
Calc Applications Abbrev：E IF IHC
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa35-53 from human MyoD family inhibitor domain-containing protein.
