Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway.
Plays an important role in the cascades of cellular responses evoked by changes in the environment.
Mediates signaling for determination of cell fate such as differentiation and survival.
Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation.
MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens.
Mediates signal transduction of various stressors like oxidative stress as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) or lipopolysaccharide (LPS).
Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7.
These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs).
Both p38 MAPK and JNKs control the transcription factors activator protein-1 (AP-1).
Applications:Suitable for use in FLISA.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes human MAP3K5.
Isotype：IgG
Calc Applications Abbrev：FLISA
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa363-431 from human mitogen-activated protein kinase kinase kinase 5 protein.
