84-5941-85　LIN28B 100ug　567281

特徴

• Suppressor of microRNA (miRNA) biogenesis, including that of let-7 andpossibly of miR107, miR-143 and miR-200c.
• Binds primary let-7 transcripts (prilet-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA (PubMed:22118463).
• Does not act on pri-miR21 (PubMed:22118463).
• The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation.
• When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation (PubMed:19703396).
• This activity might notbe relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11-independent (PubMed:22118463).
• Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop.
• Mediates MYC-induced let-7 repression (By similarity).
• When overexpressed, isoform 1 stimulates growth of the breast adenocarcinoma cell line MCF-7.
• Isoform 2 has no effect on cell growth.
• Applications:Suitable for use in ELISA, Immunohistochemistry and Immunofluorescence.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry: 1:20-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.