Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and is involved in the formation of various organs.
Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes.
In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal.
Its function as activator of the Wnt signaling pathway is required for the development of various organs, including liver, kidney, intestine, bone, reproductive tract and eye.
May also act as a receptor for norrin (NDP), such results however require additional confirmation in vivo.
Required during spermatogenesis to activate the Wnt signaling pathway in peritubular myoid cells.
Required for the maintenance of intestinal stem cells and Paneth cell differentiation in postnatal intestinal crypts.
Acts as a regulator of bone formation and remodeling.
Involved in kidney development; required for maintaining the ureteric bud in an undifferentiated state.
Involved in the development of the anterior segment of the eye.
Required during erythropoiesis.
Also acts as a negative regulator of innate immunity by inhibiting TLR2/TLR4 associated pattern-recognition and proinflammatory cytokine production.
Plays an important role in regulating the circadian rhythms of plasma lipids, partially through regulating the rhythmic expression of MTTP (By similarity).
Applications:Suitable for use in ELISA, Immunohistochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:20-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Specificity：Recognizes human LGR4.
Isotype：IgG
Calc Applications Abbrev：E IF IHC
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa234-365 from human leucine-rich repeat-containing G-protein coupled receptor 4 protein.
