84-5936-28　LATS1 100ug　567005

特徴

• Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis.
• The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ.
• Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration.
• Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint.
• Negatively regulates G2/M transition by down-regulating CDK1 kinase activity.
• Involved in the control of p53 expression.
• Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1.
• May also play a role in endocrine function.
• Plays a role in mammary gland epithelial cells differentiation, both through the Hippo signaling pathway and the intracellular estrogen receptor signaling pathway by promoting the degradation of ESR1
• Applications:Suitable for use in ELISA, Immunohistochemistry, Immunofluorescence and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry: 1:100-1:200Immunoflourescence: 1:50-1:500Immunoprecipitation: 1:200-1:2000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.