84-5917-92　JARID2 50ug　566097

特徴

• Regulator of histone methyltransferase complexes that plays an essential role in embryonic development, including heart and liver development, neural tube fusion process and hematopoiesis.
• Acts by modulating histone methyltransferase activity and promoting the recruitment of histone methyltransferase complexes to their target genes.
• Binds DNA and mediates the recruitment of the PRC2 complex to target genes in embryonic stem cells.
• Does not have histone demethylase activity but regulates activity of various histone methyltransferase complexes.
• In embryonic stem cells, it associates with the PRC2 complex and inhibits trimethylation of 'Lys-27' of histone H3 (H3K27me3) by the PRC2 complex, thereby playing a key role in differentiation of embryonic stem cells and normal development.
• In cardiac cells, it is required to repress expression of cyclin-D1 (CCND1) by activating methylation of 'Lys-9' of histone H3 (H3K9me) by the GLP1/EHMT1 and G9a/EHMT2 histone methyltransferases.
• Also acts as a transcriptional repressor of ANF via its interaction with GATA4 and NKX2-5.
• Participates in the negative regulation of cell proliferation signaling.
• Applications:Suitable for use in ELISA, Immunohistochemistry and Immunofluorescence.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry: 1:20-1:200Immunoflourescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.