Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen.
Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen.
Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin.
Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin.
Integrin alpha-5/beta-1 is a receptor for fibrinogen.
Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin.
Integrin alpha-4/beta-1 is a receptor for VCAM1 and recognizes the sequence Q-I-D-S in VCAM1.
Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin.
It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin
Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4.
Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion.
Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration.
Integrin alpha-V/beta-1 is a receptor for vitronectin.
Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands.
When associated with alpha-7/beta-1 integrin, regulates cell adhesion and laminin matrix deposition.
Involved in promoting endothelialcell motility and angiogenesis.
Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process and the formation of mineralized bone nodules.
May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1.
Together with KRT1 and RACK1, serves as a platform for SRC activation or inactivation.
Plays a mechanistic adhesive role during telophase, required for the successful completion of cytokinesis.
ITGA4:ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (By similarity).
ITGA4:ITGB1 and ITGA5:ITGB1 bind to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (By similarity).
Applications:Suitable for use in ELISA, Western Blot and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Specificity：Recognizes mouse Itgb1. Species Crossreactivity: human
Isotype：IgG
Calc Applications Abbrev：E IF WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant protein corresponding to aa21-728 from mouse Integrin beta-1 protein.
