Key transcriptional regulator of type I interferon (IFN)-dependent immuneresponses and plays a critical role in the innate immune response against DNA and RNA viruses.
Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferonstimulated response element (ISRE) in their promoters.
Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction via both the virus-activated, MyD88-independent pathway and the TLR-activated, MyD88-dependent pathway.
Required during both the early and late phases of the IFN gene induction but is more critical for the late than for the early phase.
Exists in an inactive form in the cytoplasm of uninfected cells andfollowing viral infection, double-stranded RNA (dsRNA), or toll-like receptor(TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases.
Thisinduces a conformational change, leading to its dimerization and nuclearlocalization where along with other coactivators it can activate transcription of the type I IFN and ISG genes.
Can also play a role in regulating adaptiveimmune responses by inducing PSMB9/LMP2 expression, either directly orthrough induction of IRF1.
Binds to the Q promoter (Qp) of EBV nuclear antigen 1 a (EBNA1) and may play a role in the regulation of EBV latency.
Can activate distinct gene expression programs in macrophages and regulate the anti-tumor properties of primary macrophages.
Applications:Suitable for use in ELISA, Immunohistochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:20-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Specificity：Recognizes human IRF7.
Isotype：IgG
Calc Applications Abbrev：E IF IHC
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa131-289 from human Interferon regulatory factor 7 protein.
