Key transcriptional regulator of type I interferon (IFN)-dependent immuneresponses which plays a critical role in the innate immune response against DNA and RNA viruses.
Regulates the transcription of type I IFN genes (IFNalpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferonstimulated response element (ISRE) in their promoters.
Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction.
Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases.
This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), acomplex which activates the transcription of the type I IFN and ISG genes.
Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Specificity：Recognizes mouse Irf3.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to aa1-419 from mouse Interferon regulatory factor 3 protein.
