Receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1).
Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity.
The activated IGF1R is involved in cell growth and survival control.
IGF1R is crucial for tumor transformation and survival of malignant cell.
Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins.
Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways.
Applications:Suitable for use in ELISA and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:200-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Specificity：Recognizes human IGF1R.
Isotype：IgG
Calc Applications Abbrev：E IF
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa763-931 from human Insulin-like growth factor 1 receptor protein.
