Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin.
HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production.
May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin.
Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space.
Regulates the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins.
Inhibits signaling mediated by TGF-beta family members.
This activity requires the integrity of the catalytic site, although it is unclear whether TGF-beta proteins are themselves degraded.
By acting on TGF-beta signaling, may regulate many physiological processes, including retinal angiogenesis and neuronal survival and maturation during development.
Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets.
Applications:Suitable for use in FLISA.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes human HTRA1.
Isotype：IgG
Calc Applications Abbrev：FLISA
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa23-248 from human serine protease HTRA1 protein.
