Endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans.
Participates in extracellular matrix (ECM) degradation and remodeling.
Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group.
Can also cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety.
It is essentially inactive at neutral pH but becomes active under acidic conditions such as during tumor invasion and in inflammatory processes.
Facilitates cell migration associated with metastasis, wound healing and inflammation.
Enhances shedding of syndecans, and increases endothelial invasion and angiogenesis in myelomas.
Acts as procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII.
Increases cell adhesion to the extracellular matrix (ECM), independent of its enzymatic activity.
Induces AKT1/PKB phosphorylation via lipid rafts increasing cell mobility and invasion.
Heparin increases this AKT1/PKB activation.
Regulates osteogenesis.
Enhances angiogenesis through up-regulation of SRC-mediated activation of VEGF.
Implicated in hair follicle inner root sheath differentiation and hair homeostasis.
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human HPSE.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa263-523 from human heparanase protein.
