Multifunctional protein with various roles in different cellular compartments.
May act in a redox sensitive manner.
In the nucleus is an abundant chromatinassociated non-histone protein involved in transcription, chromatin remodeling and V(D)J recombination and probably other processes.
Binds DNA with a preference to non-canonical DNA structures such as single-stranded DNA.
Can bent DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters by enhancing transcription factor binding and/or bringing distant regulatory sequences into close proximity (PubMed:7797075, PubMed:11909973, PubMed:19522541, PubMed:18413230,PubMed:19965638, PubMed:20123072).
Involved in V(D)J recombination by acting as a cofactor of the RAG complex: acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS) (By similarity).
Proposed to be involved in the innate immune response to nucleic acids by acting as a promiscuous immunogenic DNA/RNA sensor which cooperates with subsequent discriminative sensing by specific pattern recognition receptors (By similarity).
In the extracellular compartment acts as a chemokine.
Promotes proliferation and migration of endothelial cells implicating AGER/RAGE (PubMed:19811285).
Has antimicrobial activity ingastrointestinal epithelial tissues (PubMed:23877675).
Involved in inflammatory response to antigenic stimulus coupled with proinflammatory activity (By similarity).
Involved in modulation of neurogenesis probably by regulation of neural stem proliferation (By similarity).
Involved in articular cartilage surface maintenance implicating LEF1 and the Wnt/beta-catenin pathway (By similarity).
Applications:Suitable for use in ELISA, Immunohistochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:20-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Specificity：Recognizes human HMGB2.
Isotype：IgG
Calc Applications Abbrev：E IF IHC
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa43-185 from human high mobility group protein B2 protein.
