Inhibits cell growth and promotes apoptosis through the activation of p53/TP53 both at the transcription level and at the protein level (by phosphorylation and indirect acetylation).
The phosphorylation of p53/TP53 may be mediated by a p53/TP53-HIPK2-AXIN1 complex.
Involved in the response to hypoxia by acting as a transcriptional co-suppressor of HIF1A.
Mediates transcriptional activation of TP73.
In response to TGFB, cooperates with DAXX to activate JNK.
Negative regulator through phosphorylation and subsequent proteasomal degradation of CTNNB1 and the antiapoptotic factor CTBP1.
In the Wnt/beta-catenin signaling pathway acts as an intermediate kinase between MAP3K7/TAK1 and NLK to promote the proteasomal degradation of MYB.
Phosphorylates CBX4 upon DNA damage and promotes its E3 SUMO-protein ligase activity.
Activates CREB1 and ATF1 transcription factors by phosphorylation in response to genotoxic stress.
In response to DNA damage, stabilizes PML by phosphorylation.
PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53-dependent transactivation.
Promotes angiogenesis, and is involved in erythroid differentiation, especially during fetal liver erythropoiesis.
Phosphorylation of RUNX1 and EP300 stimulates EP300 transcription regulation activity.
Triggers ZBTB4 protein degradation in response to DNA damage.
Modulates HMGA1 DNA-binding affinity.
In response to high glucose, triggers phosphorylation-mediated subnuclear localization shifting of PDX1.
Involved in the regulation of eye size, lens formation and retinal lamination during late embryogenesis.
Applications:Suitable for use in ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Specificity：Recognizes human HIPK2.
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa816-977 from human homeodomain-interacting protein kinase 2 protein.
