84-5889-33　Hepatitis B virus genotype D subtype ayw Protein X (HRP) 100ug　564681

特徴

• Multifunctional protein that plays a role in silencing host antiviral defenses and promoting viral transcription.
• Does not seem to be essential for HBV infection.
• May be directly involved in development of cirrhosis and liver cancer (hepatocellular carcinoma).
• Most of cytosolic activities involve modulation of cytosolic calcium.
• The effect on apoptosis is controversial depending on the cell types in which the studies have been conducted.
• May induce apoptosis by localizing in mitochondria and causing loss of mitochondrial membrane potential.
• May also modulate apoptosis by binding host CFLAR, a key regulator of the death-inducing signaling complex (DISC).
• Promotes viral transcription by using the host E3 ubiquitin ligase DDB1 to target the SMC5-SMC6 complex to proteasomal degradation.
• This host complex would otherwise bind to viral episomal DNA, and prevents its transcription.
• Moderately stimulates transcription of many different viral and cellular transcription elements.
• Promoters and enhancers stimulated by HBx contain DNA binding sites for NF-kappa-B, AP-1, AP-2, c-EBP, ATF/CREB, or the calcium-activated factor NF-AT.
• Applications:Suitable for use in ELISA.
• Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.