84-5883-45　GSK3B 100ug　564390

特徴

• Constitutively active protein kinase that acts as a negative regulator in thehormonal control of glucose homeostasis, Wnt signaling and regulation oftranscription factors and microtubules, by phosphorylating and inactivatingglycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC,AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU and MACF1
• Requires primed phosphorylation of the majority of its substrates.
• In skeletal muscle, contributes to insulin regulation of glycogen synthesis byphosphorylating and inhibiting GYS1 activity and hence glycogen synthesis
• May also mediate the development of insulin resistance by regulating activation of transcription factors.
• Regulates protein synthesis by controlling the activity of initiation factor 2B (EIF2BE/EIF2B5) in the same manner as glycogen synthase.
• In Wnt signaling, GSK3B forms a multimeric complex with APC, AXIN1 and CTNNB1/beta-catenin and phosphorylates the N-terminal of CTNNB1 leading to its degradation mediated by ubiquitin/proteasomes.
• Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA
• Phosphorylates NFATC1/NFATC on conserved serine residues promotingNFATC1/NFATC nuclear export, shutting off NFATC1/NFATC gene regulation, and thereby opposing the action of calcineurin.
• Phosphorylates MAPT/TAU on 'Thr-548', decreasing significantly MAPT/TAU ability to bind and stabilize microtubules.
• MAPT/TAU is the principal component of neurofibrillary tangles in Alzheimer disease.
• Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex.
• Phosphorylates MACF1, inhibiting its binding to microtubules which is critical for its role in bulge stem cell migration and skin wound repair.
• Probably regulates NF-kappa-B (NFKB1) at the transcriptionallevel and is required for the NF-kappa-B-mediated anti-apoptotic response to TNF-alpha (TNF/TNFA).
• Negatively regulates replication in pancreatic betacells, resulting in apoptosis, loss of beta-cells and diabetes.
• Throughphosphorylation of the anti-apoptotic protein MCL1, may control cell apoptosis in response to growth factors deprivation.
• Phosphorylates MUC1 in breast cancer cells, decreasing the interaction of MUC1 with CTNNB1/beta-catenin.
• Is necessary for the establishment of neuronal polarity and axon outgrowth.
• Phosphorylates MARK2, leading to inhibit its activity.
• Phosphorylates SIK1 at 'Thr-182', leading to sustain its activity.
• Phosphorylates ZC3HAV1 which enhances its antiviral activity.
• Phosphorylates SNAI1, leading to its BTRCtriggered ubiquitination and proteasomal degradation.
• Phosphorylates SFPQ at 'Thr-687' upon T-cell activation.
• Phosphorylates NR1D1 st 'Ser-55' and 'Ser-59' 2and stabilizes it by protecting it from proteasomal degradation.
• Regulates the circadian clock via phosphorylation of the major clock components including ARNTL/BMAL1, CLOCK and PER2.
• Phosphorylates CLOCK AT 'Ser-427' and targets it for proteasomal degradation.
• Phosphorylates ARNTL/BMAL1 at 'Ser-17' and 'Ser-21' and primes it for ubiquitination and proteasomal degradation.
• Phosphorylates OGT at 'Ser-3' or 'Ser-4' which positively regulates its activity.
• Phosphorylates MYCN in neuroblastoma cells which may promote its degradation (PubMed:24391509).
• Applications:Suitable for use in ELISA, Western Blot and Immunofluorescence.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:5000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.