84-5875-82　GPER1 50ug　564013

特徴

• G-protein coupled estrogen receptor that binds to 17-beta-estradiol (E2) with high affinity, leading to rapid and transient activation of numerous intracellular signaling pathways.
• Stimulates cAMP production, calcium mobilization and tyrosine kinase Src inducing the release of heparin-bound epidermal growth factor (HB-EGF) and subsequent transactivation of the epidermal growth factor receptor (EGFR), activating downstream signaling pathways such as PI3K/Akt and ERK/MAPK.
• Mediates pleiotropic functions among others in the cardiovascular, endocrine, reproductive, immune and central nervous systems.
• Has a role in cardioprotection by reducing cardiac hypertrophy and perivascular fibrosis in a RAMP3-dependent manner.
• Regulates arterial blood pressure by stimulating vasodilation and reducing vascular smooth muscle and microvascular endothelial cell proliferation.
• Plays a role in blood glucose homeostasis contributing to the insulin secretion response by pancreatic beta cells.
• Triggers mitochondrial apoptosis during pachytene spermatocyte differentiation.
• Stimulates uterine epithelial cell proliferation.
• Enhances uterine contractility in response to oxytocin.
• Contributes to thymic atrophy by inducing apoptosis.
• Attenuates TNF-mediated endothelial expression of leukocyte adhesion molecules.
• Promotes neuritogenesis in developing hippocampal neurons.
• Plays a role in acute neuroprotection against NMDA-induced excitotoxic neuronal death.
• Increases firing activity and intracellular calcium oscillations in luteinizing hormone-releasing hormone (LHRH) neurons.
• Inhibits early osteoblast proliferation at growth plate during skeletal development.
• Inhibits mature adipocyte differentiation and lipid accumulation.
• Involved in the recruitment of beta-arrestin 2 ARRB2 at the plasma membrane in epithelial cells.
• Functions also as a receptor for aldosterone mediating rapid regulation of vascular contractibility through the PI3K/ERK signaling pathway.
• Involved in cancer progression regulation.
• Stimulates cancer-associated fibroblast (CAF) proliferation by a rapid genomic response through the EGFR/ERK transduction pathway.
• Associated with EGFR, may act as a transcription factor activating growth regulatory genes (c-fos, cyclin D1).
• Promotes integrin alpha-5/beta-1 and fibronectin (FN) matrix assembly in breast cancer cells.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:5000Immunohistochemistry: 1:20-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.