84-5874-69　GP (Biotin) 50ug　563957

特徴

• GP1 is responsible for binding to the receptor(s) on target cells.
• Interacts with CD209/DC-SIGN and CLEC4M/DC-SIGNR which act as cofactors for virus entry into the host cell.
• Binding to CD209 and CLEC4M, which are respectively found on dendritic cells (DCs), and on endothelial cells of liver sinusoids and lymph node sinuses, facilitate infection of macrophages and endothelial cells.
• These interactions not only facilitate virus cell entry, but also allow capture of viral particles by DCs and subsequent transmission to susceptible cells without DCs infection (trans infection).
• Binding to the macrophage specific lectin CLEC10A also seem to enhance virus infectivity.
• Interaction with FOLR1/folate receptor alpha may be a cofactor for virus entry in some cell types, although results are contradictory.
• Members of the Tyro3 receptor tyrosine kinase family also seem to be cell entry factors in filovirus infection.
• Once attached, the virions are internalized through clathrin-dependent endocytosis and/or macropinocytosis.
• After internalization of the virus into the endosomes of the host cell, proteolysis of GP1 by two cysteine proteases, CTSB/cathepsin B and CTSL/cathepsin L presumably induces a conformational change of GP2, allowing its binding to the host entry receptor NPC1 and unmasking its fusion peptide to initiate membranes fusion.
• Applications:Suitable for use in ELISA.
• Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.