84-5865-39 gelE 100ug 563496
特徴
- Metalloprotease capable of the hydrolysis of insoluble hydrophobic substrates.
- Hydrolyzes azocoll and gelatin and, at a lower rate, soluble and insoluble collagens.
- Does not cleave short synthetic peptides.
- Preferentially hydrolyzes the 24-Phe-|-Phe-25 bond in the insulin B-chain, followed by the 5-His-|-Leu-6 bond.
- Inactivates endothelin-1, primarily by cleavage of the 5-Ser-|-Leu-6 and 16-His-|-Leu-17 bonds.
- Hydrolyzes the alpha chain of C3 to generate a C3b-like protein.
- Inhibits complement-mediated hemolysis and opsinization of bacteria.
- Hydrolyzes the insect antimicrobial peptide cecropin.
- Decreases the length of E.faecalis chains via the activation of autolysin.
- Degrades polymerized fibrin.
- Applications:Suitable for use in ELISA and Western Blot.
- Other applications not tested.
- Recommended Dilution:Western Blot: 1:500-1:5000Optimal dilutions to be determined by the researcher.
- Storage and Stability:May be stored at 4°C for short-term only.
- Aliquot to avoid repeated freezing and thawing.
- Store at -20°C.
- Aliquots are stable for 12 months after receipt.
- For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
- Size：100ug
- Host：rabbit
- Source Antibody：Enterococcus faecalis
- Grade：Affinity Purified
- Purity：Purified by Protein G affinity chromatography.
- Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
- Specificity：Recognizes Enterococcus faecalis gelE.
- Isotype：IgG
- Calc Applications Abbrev：E WB
- Immunogen：Recombinant protein corresponding to aa193-510 from enterococcus faecalis gelatinase protein.
- Shelf Life：1year
- Swiss Prot Number：Q833V7
- EU Commodity Code：30021010
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