Transcription factor that is the main target of insulin signaling and regulatesmetabolic homeostasis in response to oxidative stress.
Binds to the insulinresponse element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'-TT[G/A]TTTAC-3'.
Activity suppressed by insulin.
Main regulator of redox balance and osteoblast numbers and controls bone mass.
Orchestrates the endocrine function of the skeleton in regulating glucose metabolism.
Acts synergistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity.
Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP.
In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A and CEBPA to activate the expression of genes suchas IGFBP1, G6PC and PCK1.
Important regulator of cell death actingdownstream of CDK1, PKB/AKT1 and SKT4/MST1.
Promotes neural cell death.
Mediates insulin action on adipose tissue.
Regulates the expression of adipogenic genes such as PPARG during preadipocyte differentiation and, adipocyte size and adipose tissue-specific gene expression in response to excessive calorie intake.
Regulates the transcriptional activity of GADD45A and repair of nitric oxide-damaged DNA in beta-cells.
Required for the autophagic cell death induction in response to starvation or oxidative stress in a transcription-independent manner.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:5000Immunohistochemistry: 1:20-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Specificity：Recognizes human FOXO1. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant protein corresponding to aa1-300 from human Forkhead box protein O1 protein.
