During virus entry, induces fusion of viral and cellular membranes leading to delivery of the nucleocapsid into the cytoplasm.
The fusogenic activity is inactive untill entry into host cell endosome, where a furin-like protease cleaves off a small peptide between F1 and F2 (PubMed:18216092).
Interacts directly with heparan sulfate and may participates in virus attachment (PubMed:10864656).
Furthermore, the F2 subunit was identifed as the major determinant of RSV host cell specificity (PubMed:11493675).
Later in infection, proteins F expressed atthe plasma membrane of infected cells can mediate fusion with adjacent cells to form syncytia, a cytopathic effect that could lead to tissue necrosis.
The fusion protein is also able to trigger p53-dependent apoptosis (PubMed:12663767).
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Specificity：Recognizes human F.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa27-529 from human respiratory syncytial virus A fusion glycoprotein F0 protein.
