Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides.
Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules.
Strongly prefers substrates 9-16 residues long.
Rapidly degrades 13-mer to a 9-mer and then stops.
Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminal, while it has weak activity toward peptides with charged C-terminal.
May play a role in the inactivation of peptide hormones.
May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000Immunohistochemistry: 1:20-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Specificity：Recognizes human ERAP1.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa642-941 from human endoplasmic reticulum aminopeptidase 1 protein.
