Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication.
The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase.
E2F1 binds preferentially RB1 in a cell-cycle dependent manner.
It can mediate both cell proliferation and TP53/p53-dependent apoptosis.
Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters.
Applications:Suitable for use in FLISA.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes human E2F1.
Isotype：IgG
Calc Applications Abbrev：FLISA
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa133-362 from human transcription factor E2F1 protein.
