Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development.
DNA methylation is coordinated with methylation of histones.
May preferentially methylates nucleosomal DNA within the nucleosome core region.
May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation.
Seems to be involved in gene silencing (By similarity).
In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.
Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs.
Function as transcriptional corepressor by associating with ZHX1.
Required for DUX4 silencing in somatic cells.
Applications:Suitable for use in FLISA.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes human DNMT3B.
Isotype：IgG
Calc Applications Abbrev：FLISA
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa1-100 from human DNA (cytosine-5)-methyltransferase 3B protein.
