Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development.
DNA methylation is coordinated with methylation of histones.
May preferentially methylates nucleosomal DNA within the nucleosome core region.
May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation.
Seems to be involved in gene silencing (By similarity).
In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.
Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs.
Function as transcriptional corepressor by associating with ZHX1.
Required for DUX4 silencing in somatic cells
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:5000Immunohistochemistry: 1:20-1:200Immunoflourescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Specificity：Recognizes human DNMT3B.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa1-100 from human DNA (cytosine-5)-methyltransferase 3B protein.
