Transcriptional activator involved in gene imprinting.
Catalyzes the release of 5-methylcytosine (5-meC) from DNA by a glycosylase/lyase mechanism.
Allows the expression of the maternal copy of the imprinted MEA gene before fertilization, possibly by antagonizing or suppressing DNA methylation on target promoter.
Probably acts by nicking the MEA promoter.
Required for stable reproducible patterns of floral and vegetative development.
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：Arabidopsis thaliana
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes arabidopsis thaliana DME.
Isotype：IgG
Calc Applications Abbrev：E
Immunogen：Recombinant protein corresponding to aa1-320 from arabidopsis thaliana transcriptional activator DEMETER.
