84-5788-76　CXCL12 100ug　559703

特徴

• Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils.
• Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis.
• Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1.
• SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity.
• Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites.
• Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase.
• Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins.
• SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase.
• Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1.
• Plays a protective role after myocardial infarction.
• Induces down-regulation and internalization of ACKR3 expressed in various cells.
• Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation.
• Applications:Suitable for use in Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry: 1:50-300ELISA: 1:10,000-20,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.