84-5787-71　CUL4A 100ug　559651

特徴

• Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination of target proteins.
• As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme.
• The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1.
• The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component.
• DCX(DET1-COP1) directs ubiquitination of JUN.
• DCX(DDB2) directs ubiquitination of XPC.
• DCX(DDB2) ubiquitinates histones H3-H4 and is required for efficient histone deposition during replication-coupled (H3.1) and replication-independent (H3.3) nucleosome assembly, probably by facilitating the transfer of H3 from ASF1A/ASF1B to other chaperones involved in histone deposition.
• DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication.
• In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip.
• Is involved in ubiquitination of HOXA9.
• DCX(DTL) directs autoubiquitination of DTL.
• Applications:Suitable for use in ELISA and Immunofluorescence.
• Other applications not tested.
• Recommended Dilution:Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.